primary antibodies against crt  (R&D Systems)

Journal: Oncology Letters

Article Title: Significance of calreticulin as a prognostic factor in endometrial cancer

doi: 10.3892/ol.2018.8495

Figure Lengend Snippet: Representative photomicrographs of immunohistochemical staining of endometrial cancer specimens with anti-CRT, p-eIF2a, and Ki67 antibody. Immunostaining for (A) CRT, (B) p-eIF2a, and (C) Ki67 in endometrial cancer cells. CRT, calreticulin; p-eIF2α, phosphorylated eukaryotic initiation factor 2α.

Article Snippet: Primary antibodies against CRT (MAB3898), p-eIF2a (AF705) and PERK (AF3999) were purchased from R&D systems.

Techniques: Immunohistochemical staining, Staining, Immunostaining

Journal: Oncology Letters

Article Title: Significance of calreticulin as a prognostic factor in endometrial cancer

doi: 10.3892/ol.2018.8495

Figure Lengend Snippet: Assessment of CRT, PERK, eIF2α, and p-eIF2α protein content by Western blot analysis. The protein content of CRT, PERK, eIF2α, and p-eIF2α in 12 pairs of DOX-resistant endometrial cancer patients and DOX-sensitive endometrial cancer patients was assessed. In the DOX-resistant endometrial cancer samples, the CRT, PERK, eIF2α, and p-eIF2α protein content was not significantly different between the pre-NAC and post-NAC. (A) In the DOX-sensitive endometrial cancer samples, the CRT, PERK, and p-eIF2α protein content was significantly increased in the post-NAC group compared with the pre-NAC group. (C) There was not a significant change in the eIF2α protein content in the DOX-sensitive endometrial cancer samples. The expression levels were normalized to the actin/pan-cadherin expression values. (B and D) The data are expressed as the means ± standard deviations, which were calculated from three parallel experiments. *P<0.05. CRT; calreticulin; PERK, PKR-like endoplasmic reticulum kinase.

Article Snippet: Primary antibodies against CRT (MAB3898), p-eIF2a (AF705) and PERK (AF3999) were purchased from R&D systems.

Techniques: Western Blot, Expressing

Journal: Molecular Therapy Oncolytics

Article Title: Anti-tumor immunity enhancement by photodynamic therapy with talaporfin sodium and anti-programmed death 1 antibody

doi: 10.1016/j.omto.2022.12.009

Figure Lengend Snippet: Induction of damage-associated molecular patterns (DAMPs) by TS-PDT in vitro (A) Flow cytometric analysis of cell-surface calreticulin (CRT). Negative controls, without TS addition or irradiation, are shown in red. The treated groups are shown in blue. (B) CRT translocation from the endoplasmic reticulum (ER) to the cell surface assessed by immunofluorescence staining. Images were obtained using a confocal microscope (original magnification ×350; scale bar, 5 μm). (C) Extracellular release of high-mobility group protein B1 (HMGB1) induced by TS-PDT assessed by the enzyme-linked immunosorbent assay (ELISA) (n = 3). (D) HMGB1 translocation from the ER to the cell surface was assessed by immunofluorescence staining. Images were obtained using a confocal microscope (original magnification ×350; scale bar, 5 μm). (E) Heat-shock protein (HSP)-90 expression on the cell surface induced by TS-PDT assessed by flow cytometry. Negative controls, without TS addition nor irradiation, are shown in red. The treated groups are shown in blue. (F) Extracellular release of ATP induced by TS-PDT assessed by ELISA. ATP concentration in the culture medium after TS-PDT was detected by ELISA (n = 3).

Article Snippet: Primary antibodies against CRT (1:1,000 dilution; ab2907; Abcam) or HMGB1 (1:250 dilution; ab79823; Abcam) were used.

Techniques: In Vitro, Irradiation, Translocation Assay, Immunofluorescence, Staining, Microscopy, Enzyme-linked Immunosorbent Assay, Expressing, Flow Cytometry, Concentration Assay